Insecticide Resistance in Natural Populations of Domestic Flies
Comprehensive doctoral thesis examining insecticide resistance in natural populations of Musca domestica (domestic fly). This work presents detailed genetic and biochemical aspects of resistance mechanisms, including DDT resistance through KDR genes and metabolic pathways, resistance to organophosphates and carbamates through altered cholinesterase and enzymatic detoxification. The study covers biochemical and genetic investigation techniques, resistance due to reduced insecticide absorption, and metabolic resistance involving dehydrochlorinase, mixed-function oxidases, hydrolases, and glutathione S-transferase. Extensive experimental testing of fly strains from various geographic origins, solvent effects on insecticide toxicity, and statistical analysis of resistance distribution patterns. Includes comprehensive literature review and detailed discussion of resistance manifestation mechanisms in field populations, providing essential reference material for entomological pest management and insecticide efficacy studies.
Table of Contents:
- Foreword .... 1
- INTRODUCTION - Factors influencing insecticide resistance in natural populations of domestic flies .... 3
- CHAPTER I - Genetic and biochemical aspects of resistance in Musca domestica L. .... 11
- 1 - Study techniques .... 13
- 1.1. Biochemical techniques .... 13
- 1.2. Genetic techniques .... 16
- 2 - Resistance due to reduced absorption of insecticides .... 17
- 3 - Resistance to DDT .... 18
- 3.1. Resistance due to the KDR gene .... 18
- 3.2. Resistance due to metabolism .... 20
- 3.2.1. Resistance due to dehydrochlorinase .... 20
- 3.2.2. Resistance due to mixed function oxidases .... 22
- 4 - Resistance to organophosphates and carbamates .... 24
- 4.1. Altered cholinesterase .... 24
- 4.2. Resistance due to metabolism .... 26
- 4.2.1. Hydrolases and glutathione-dependent transferases .... 26
- 4.2.1.1. Hydrolases .... 27
- 4.2.1.2. Glutathione S-transferase .... 33
- 4.2.2. Mixed function oxidases .... 34
- 4.2.2.1. Organophosphates .... 35
- 4.2.2.2. Carbamates .... 36
- 5 - Conclusion .... 39
- CHAPTER II - Study of insecticide resistance in domestic flies of various geographical origins .... 41
- 1 - Origin and rearing of strains .... 43
- 1.1. Geographical origin .... 43
- 1.2. Laboratory rearing of flies .... 44
- 1.3. Statistical study of weight homogeneity of female flies .... 46
- 2 - Insecticides used .... 48
- 2.1. Mode of action .... 48
- 2.2. Formulas and physico-chemical properties of insecticides .... 49
- 2.3. Dilutions .... 54
- 3 - Test execution .... 55
- 3.1. Composition of test equipment .... 55
- 3.2. Technique used .... 56
- 3.3. Role of solvent in insecticide toxicity for domestic flies .... 57
- 3.3.1. Tests .... 58
- 3.3.2. Results .... 66
- 4 - Results and interpretations of tests .... 79
- 4.1. Results .... 79
- 4.2. Interpretation of tests .... 79
- 4.3. Discussion .... 138
- 5 - Conclusion .... 141
- 5.1. Distribution of resistances .... 141
- 5.2. Modes of manifestation of these resistances .... 150
- Bibliography .... 155
- Abstract (French) .... 170
- Summary .... 173
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Document Details
- Format
- Pages
- 180
- Size
- 2.3 MB
- Category
- Society & Culture